GDP-6-Alkyne-Fuc

Guanosine 5′-diphospho-β-6-alkyne-L-fucose disodium salt (GDP-β-6-alkyne-L-fucose) is a synthetic nucleotide sugar analog. It is based on GDP-L-fucose, which is involved in fucosylation, a glycosylation process where fucose is added to glycoproteins and glycolipids.

Key Features:

• GDP-L-Fucose Analogue: GDP-L-fucose is a donor substrate used by fucosyltransferases in the fucosylation of glycans, contributing to the biosynthesis of complex glycoconjugates.
• 6-Alkyne Modification: The alkyne group at the 6th position of the fucose sugar provides a reactive handle for bioorthogonal click chemistry. This enables researchers to tag and visualize fucosylated structures in cells using azide-alkyne cycloaddition (“click chemistry”).
• Disodium Salt: The disodium salt form increases the stability and solubility of the molecule in aqueous solutions, which is beneficial for biochemical experiments.

Applications:

This compound is useful in metabolic labeling and tracking fucosylation in glycomics studies. It allows for the incorporation of a reactive alkyne group into fucosylated glycans, which can later be conjugated to fluorescent or affinity tags for visualization and isolation. This makes it valuable for studying glycosylation pathways and for developing fucose-based therapeutics.

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