Case 01
N-glycan synthesis at gram scale
Bottom-up assembly through a fully enzymatic route
- Project quantities
- 1–10 g
- Specified purity
- ≥95%
- Stereochemical control
- Enzymatic
The brief
The project called for gram-scale Asn-linked N-glycans across multiple glycosylation patterns, with defined structures and a purity specification of at least 95%.
Our approach
A bottom-up, fully enzymatic synthesis strategy used the selectivity of glycosyltransferases to control glycosidic bond formation and achieve high stereochemical control during assembly.
Project scope
The project scope included both 1 g and 10 g N-glycan requirements. Each target was specified separately to align structure, quantity, and purity with the research program.
