3-Indolyl phosphate disodium salt; 3-Indoxyl phosphate disodium salt
Histochemical substrate for alkaline phosphatase.
3-Indoxyl phosphate is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate has been used in ELISAs.
Indol-3-yl sodium phosphate is a white to off-white powder. (NTP, 1992)
| CAS Number | 3318-43-2 |
| Product Name | Indol-3-yl sodium phosphate |
| IUPAC Name | disodium;1H-indol-3-yl phosphate |
| Molecular Formula | C8H6NNa2O4P |
| Molecular Weight | 257.09 g/mol |
| InChI | InChI=1S/C8H8NO4P.2Na/c10-14(11,12)13-8-5-9-7-4-2-1-3-6(7)8;;/h1-5,9H,(H2,10,11,12);;/q;2*+1/p-2 |
| InChI Key | WCSKWBKPKXJWEG-UHFFFAOYSA-L |
| SMILES | C1=CC=C2C(=C1)C(=CN2)OP(=O)([O-])[O-].[Na+].[Na+] |
| Solubility | greater than or equal to 100 mg/mL at 72? F (NTP, 1992) |
| Synonyms | 3-Indolyl Phosphate Disodium Salt; 3-Indoxyl Phosphate Disodium Salt; |
| Canonical SMILES | C1=CC=C2C(=C1)C(=CN2)OP(=O)([O-])[O-].[Na+].[Na+] |
| Size | 1 G, 100 MG, 5 G, Other |
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