b1,3-GalNAc transferase (LgtD)

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Nameβ1,3-N-acetylgalactosaminyltransferase:LgtD
Catalog NumberEN01007
E.C.2.4.1.79
Product DescriptionE. coli recombinant β1,3-N-acetylgalactosami-nyltransferase from Neisseria meningitides
Unit DefinitionOne unit is defined as the amount of enzyme that catalyzes the formation of 1 µmol of GalNAcβ1,3-Gal from UDP-GalNAc and Gal per min at 37 °C.
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b1,3-GalNAc transferase (LgtD)

Accepted Name
Globotriaosylceramide 3-beta-N-acetylgalactosaminyltransferase.
Alternative Name(s)
Beta-3GalNAc-T1.
Galactosylgalactosylglucosylceramide beta-D- acetylgalactosaminyltransferase.
Globoside synthase.
Globoside synthetase.
Uridine diphosphoacetylgalactosamine-galactosylgalactosylglucosylceramide acetylgalactosaminyltransferase.
Reaction catalysed
UDP-N-acetyl-alpha-D-galactosamine + alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide <=> UDP + N-acetyl-beta-D-galactosaminyl-(1->3)-alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
Cofactor(s)
Mn(2+).
Comment(s)
  • Globoside is a neutral glycosphingolipid in human erythrocytes and has blood-group-P-antigen activity.
  • UDP-GalNAc is the only sugar donor that is used efficiently by the enzyme: UDP-Gal and UDP-GlcNAc result in very low enzyme activity.
  • Lactosylceramide, globoside and gangliosides GM3 and GD3 are not substrates.

2. MSDS

3. Tech Data Sheets/Manuals

Size

10U, 1U, 5U, Other

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