Acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on asialo- alpha(1)-acid glycoprotein and N-acetyllactosamine (beta-D- galactosyl-1,4-N-acetyl-beta-D-glucosamine), but not on 2′-fucosylated-N-acetyllactosamine.
The non-reducing terminal N-acetyllactosamine residues of glycoproteins can also act as acceptor.