2-Chloro-4-nitrophenyl b-D-cellobioside
Chromogenic substrate for cellulase activity. Suitable for kinetic characterisation of cellulases. Useful for biofuels research to measure cellulase activity for optimum ethanol production.
2-Chloro-4-nitrophenyl b-D-cellobioside (CNPG) is a chromogenic enzyme substrate that is commonly used to detect and measure the activity of beta-glucosidases. CNPG is cleaved by the enzyme into the chromogenic product, which can be detected spectrophotometrically. beta-glucosidases are important enzymes in the breakdown of cellulose, and CNPG is a widely used substrate for the detection and quantification of these enzymes in various research studies.
CAS Number |
135743-28-1 |
Product Name |
2-Chloro-4-nitrophenyl-beta-D-cellobioside |
IUPAC Name |
(2S,3R,4S,5S,6R)-2-[(2R,3S,4R,5R,6S)-6-(2-chloro-4-nitrophenoxy)-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol |
Molecular Formula |
C18H24ClNO13 |
Molecular Weight |
497.834 |
InChI |
InChI=1S/C18H24ClNO13/c19-7-3-6(20(28)29)1-2-8(7)30-17-15(27)13(25)16(10(5-22)32-17)33-18-14(26)12(24)11(23)9(4-21)31-18/h1-3,9-18,21-27H,4-5H2/t9-,10-,11-,12+,13-,14-,15-,16-,17-,18+/m1/s1 |
InChI Key |
RFGBZYLCQCJGOG-KFRZSCGFSA-N |
SMILES |
C1=CC(=C(C=C1[N+](=O)[O-])Cl)OC2C(C(C(C(O2)CO)OC3C(C(C(C(O3)CO)O)O)O)O)O |
CAS No: 135743-28-1
MDL No: MFCD00064017
Chemical Formula: C18H24ClNO13
Molecular Weight: 497.83 |
References:
1. Kandra L, Gyemant G, Farkas E, Liptak A,, Carbohyd. Res., 1997, 298, p237 |