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Catalog No: A10GD-4122
Cas No: 369-07-3
Properties: Mol Formula: C12H15NO8, Mol Weight: 301.25
IUPAC Name: (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
Synonym: ONPG, 2-Nitrophenyl beta-D-galactopyranoside, o-Nitrophenyl beta-D-galactopyranoside
2-Nitrophenyl b-D-galactopyranoside – non-animal origin
o-Nitrophenyl ?-D-galactopyranoside (ONPG) is a chromogenic substrate for ?-galactosidase. Upon enzymatic cleavage by ?-galactosidase, o-nitrophenol is released, which can be quantified by colorimetric detection at 420 nm as a measure of ?-galactosidase activity.
2-nitrophenyl beta-D-galactoside is a beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position. It has a role as a chromogenic compound. It is a beta-D-galactoside and a C-nitro compound. It derives from a 2-nitrophenol.
| CAS Number | 369-07-3 |
| Product Name | 2-Nitrophenyl-beta-D-galactopyranoside |
| IUPAC Name | (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol |
| Molecular Formula | C??H??NO? |
| Molecular Weight | 301.25 g/mol |
| InChI | InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1 |
| InChI Key | KUWPCJHYPSUOFW-UHFFFAOYSA-N |
| SMILES | C1=CC=C(C(=C1)[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O |
| Solubility | 45.2 [ug/mL] |
| Synonyms | 2-nitrophenyl-alpha-D-galactoside, 2-nitrophenylgalactoside, 2-nitrophenylgalactoside, (alpha-D)-isomer, 2-nitrophenylgalactoside, (beta-D)-isomer, o-nitrophenyl-beta-D-galactopyranoside, ONPG, ONPG cpd, ortho-nitrophenyl-beta-D-galactoside |
| Canonical SMILES | C1=CC=C(C(=C1)[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O |
| Isomeric SMILES | C1=CC=C(C(=C1)[N+](=O)[O-])O[C@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O |
CAS No: 369-07-3 Synonyms: ONPG MDL No: MFCD00063255 Chemical Formula: C12H15NO8 Molecular Weight: 301.25 |
COA:
Product name: 2-Nitrophenyl-?-D-galactopyranoside
CAS: 369-07-3 M.F.: C12H15O8 M.W.: 301.25
|
Items |
Standards |
Results |
|
Appearance |
White or off-white crystal powder |
Complies |
|
Solubility |
Soluble in DMF or hot and insoluble in ether |
Complies |
|
Color and Turbidity ?in 15mg/ml in water? |
Colorless or light yellow and clear |
Comply |
|
NMR and MS |
Should comply |
Comply |
|
Specific rotation [?]20/D, in water |
-68 ~ -72? |
-70? |
|
Melting point |
186-193 ?C (Dec.) |
188-190? |
|
Carbon |
46.9 – 48.8 % |
Complies |
|
Nitrogen |
4.5 – 4.8 % |
Complies |
|
Loss weight on dryness |
Max. 0.5% |
0.11% |
|
Residue on ignition |
0.5% max |
0.2% |
|
Heavy metals |
Max. 20 ppm |
Complies |
|
Assay by HPLC |
Min. 98% |
99.2% |
|
Purity (Enzymatic) |
Min. 98% |
Complies |
References:
1. Maruhn D, Clin. Chim. Acta 1976, 73, 453
2. Levvy GA, Conchie J, Methods Enzymol. 1966, 8, 571-84
3. Naider F, et al., Biochemistry 1972, 11, 3202-7
| Size | 1 G, 100 MG, 5 G, Other |
|---|
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