4-Nitrophenyl a-D-galactopyranoside
The 4-Nitrophenyl-alpha-D-galactopyranoside is a high-quality chromogenic substrate specifically designed for the enzymatic assay of alpha-galactosidase. Upon enzyme catalysis, it yields a highly fluorescent 4-nitrophenol product, which allows easy and accurate measurement of enzyme activity in a variety of research applications, such as biochemical analysis, inhibitor screening, and enzyme kinetics studies.
CAS No: 7493-95-0
Synonyms: PNP-a-Gal
MDL No: MFCD00065050
Chemical Formula: C12H15NO8
Molecular Weight: 301.25
|
CAS Number |
7493-95-0 |
Product Name |
4-nitrophenyl-alpha-D-galactopyranoside |
IUPAC Name |
(2R,3R,4S,5R,6R)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol |
Molecular Formula |
C12H15NO8 |
Molecular Weight |
301.25 g/mol |
InChI |
InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12+/m1/s1 |
InChI Key |
IFBHRQDFSNCLOZ-IIRVCBMXSA-N |
SMILES |
C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O |
Synonyms |
4-nitrophenyl-alpha-galactoside, 4-nitrophenylgalactoside, 4-nitrophenylgalactoside, (alpha-D)-isomer, p-nitrophenyl-alpha-D-galactopyranoside, p-nitrophenyl-alpha-galactoside, para-nitrophenyl-beta-D-galactoside, PNPG |
Canonical SMILES |
C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O |
Isomeric SMILES |
C1=CC(=CC=C1[N+](=O)[O-])O[C@@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O |
COA:
name:4-Nitrophenyl-?-D-galactopyranoside
M.F.: C12H15O8 M.W.: 301.25 CAS: 7493-95-0
Items
|
Standards
|
Results
|
Appearance
|
White or slightly yellow crystal powder
|
Complies
|
Solubility
|
Soluble in DMF and insoluble in ether
|
Complies
|
NMR and MS
|
Should comply
|
Complies
|
Identification
|
IR and HPLC
|
Complies
|
Specific rotation
[?]20/D, (c=0.4, in water)
|
+218? ~ +222?
|
220?
|
Loss weight on dryness
|
Max. 1%
|
0.5%
|
Any impurity
|
Max. 0.5%
|
Complies
|
Residue on ignition
|
Max.1%
|
0.2%
|
Heavy metals
|
Max.20 ppm
|
Complies
|
TLC
|
Should be one spot
|
one spot
|
Purity
|
Min.98%
|
99.2%
|
Assay by HPLC
|
98% ~ 102%
|
99.4%
|