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4-Nitrophenyla-D-galactopyranoside

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Catalog No: A10GD-4703
Cas No: 7493-95-0
Properties: Mol Formula: C12H15NO8, Mol Weight: 301.25
IUPAC Name: (2R,3R,4S,5R,6R)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol
Synonym: 4-nitrophenyl-alpha-D-galactopyranoside, p-Nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactoside

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4-Nitrophenyl a-D-galactopyranoside

The 4-Nitrophenyl-alpha-D-galactopyranoside is a high-quality chromogenic substrate specifically designed for the enzymatic assay of alpha-galactosidase. Upon enzyme catalysis, it yields a highly fluorescent 4-nitrophenol product, which allows easy and accurate measurement of enzyme activity in a variety of research applications, such as biochemical analysis, inhibitor screening, and enzyme kinetics studies.


CAS No: 7493-95-0

Synonyms: PNP-a-Gal

MDL No: MFCD00065050

Chemical Formula: C12H15NO8

Molecular Weight: 301.25

CAS Number 7493-95-0
Product Name 4-nitrophenyl-alpha-D-galactopyranoside
IUPAC Name (2R,3R,4S,5R,6R)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol
Molecular Formula C12H15NO8
Molecular Weight 301.25 g/mol
InChI InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12+/m1/s1
InChI Key IFBHRQDFSNCLOZ-IIRVCBMXSA-N
SMILES C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O
Synonyms 4-nitrophenyl-alpha-galactoside, 4-nitrophenylgalactoside, 4-nitrophenylgalactoside, (alpha-D)-isomer, p-nitrophenyl-alpha-D-galactopyranoside, p-nitrophenyl-alpha-galactoside, para-nitrophenyl-beta-D-galactoside, PNPG
Canonical SMILES C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O
Isomeric SMILES C1=CC(=CC=C1[N+](=O)[O-])O[C@@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O

COA:

name:4-Nitrophenyl-?-D-galactopyranoside 

M.F.: C12H15O8          M.W.: 301.25                CAS: 7493-95-0

Items

Standards

Results

Appearance

White or slightly yellow crystal powder

Complies

Solubility

Soluble in DMF and insoluble in ether

Complies

NMR and MS

Should comply

Complies

Identification

IR and HPLC

Complies

Specific rotation

[?]20/D, (c=0.4, in water)

+218? ~ +222?

220?

Loss weight on dryness

Max. 1%

0.5%

Any impurity

Max. 0.5%

Complies

Residue on ignition

Max.1%

0.2%

Heavy metals

Max.20 ppm

Complies

TLC

Should be one spot

one spot

Purity

Min.98%

99.2%

Assay by HPLC

98% ~ 102%

99.4%

  1. COA

2. MSDS

3. Tech Data Sheets/Manuals

Size

1 G, 100 MG, 5 G, Other

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