5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is a high quality, enzyme substrate that is used for the detection of glucose in culture media and diagnostics. 5B4CI3AM is a chromogenic and bioluminescent substrate that can be used to detect the presence of ligands. The reaction with this compound yields a yellow color when oxidized. This product is also available as a chemiluminescent, fluorogenic, and fluorescence substrate. 5B4CI3AM has been shown to be an excellent high purity reagent for use in environmental testing and food testing.