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Catalog No: A10GD-4563
Cas No: 1708766
Properties: Mol Formula: C15H15BrClN2O4P, Mol Weight: 433.62
IUPAC Name: (5-bromo-4-chloro-1H-indol-3-yl) dihydrogen phosphate;4-methylaniline
Synonym: BCIP, BCIP p-toluidine salt, X-phosphate p-toluidine salt
BCIP is a colorimetric substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, an indoxyl intermediate is released and subsequently oxidized to produce water-insoluble indigo. It can be used to detect alkaline phosphatase activity by immunohistochemistry (IHC) or Western blot (WB).
BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity.
A substrate for visualising alkaline phosphatase activity. It produces a purple insoluble end product that is detected visually, when used together with Nitroblue tetrazolium. The substrate system is versatile and has functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs, microarray and immunohistochemistry. DMF soluble.
| CAS Number | 6578-06-9 |
| Product Name | (5-bromo-4-chloro-1H-indol-3-yl) hydrogen phosphate;(4-methylphenyl)azanium |
| IUPAC Name | (5-bromo-4-chloro-1H-indol-3-yl) hydrogen phosphate;(4-methylphenyl)azanium |
| Molecular Formula | C??H??BrClN?O?P |
| Molecular Weight | 433.62 g/mol |
| InChI | InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3 |
| InChI Key | QEIFSLUFHRCVQL-UHFFFAOYSA-N |
| SMILES | CC1=CC=C(C=C1)[NH3+].C1=CC(=C(C2=C1NC=C2OP(=O)(O)[O-])Cl)Br |
| Canonical SMILES | CC1=CC=C(C=C1)[NH3+].C1=CC(=C(C2=C1NC=C2OP(=O)(O)[O-])Cl)Br |
| Size | 1 G, 100 MG, 5 G, Other |
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IUPAC:Fuc(a1-2)[GalNAc(a1-3)]Gal(b1-3)GlcNAc(b1-3)Gal(b1-4)b-Glc, G82858KU
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