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Catalog No: A10GD-4420
Cas No: 157843-41-9
Properties: Mol Formula: C12H14ClNO7, Mol Weight: 319.69
IUPAC Name: (2S,3S,4R,5S,6S)-2-(2-chloro-4-nitrophenoxy)-6-methyloxane-3,4,5-triol
Synonym: 2-Chloro-4-nitrophenyl-alpha-L-fucopyranoside, 2-Chloro-4-nitrophenyl a-L-fucopyranoside, (2S,3S,4R,5S,6S)-2-(2-chloro-4-nitrophenoxy)-6-methyloxane-3,4,5-triol
Chromogenic substrate for alpha-L-Fucosidase. The AFU assay kit is based on the enzymatic cleavage of the synthetic substrate CNPF to ?-L-fucose and 2-chloro-4-nitrophenol, which is quantified by measuring the absorbances at 405 nm in a kinetic fashion. It is a one step assay with a single assay reagent. One unit of AFU is defined as the amount of AFU that cleaves one ?mole of 2-chloro-4-nitrophenyl-?-L-fucoside per min at 37 ?C.
| CAS Number | 157843-41-9 |
| Product Name | 2-Chloro-4-nitrophenyl-alpha-L-fucopyranoside |
| IUPAC Name | (2S,3S,4R,5S,6S)-2-(2-chloro-4-nitrophenoxy)-6-methyloxane-3,4,5-triol |
| Molecular Formula | C??H??ClNO? |
| Molecular Weight | 319.69 g/mol |
| InChI | InChI=1S/C12H14ClNO7/c1-5-9(15)10(16)11(17)12(20-5)21-8-3-2-6(14(18)19)4-7(8)13/h2-5,9-12,15-17H,1H3/t5-,9+,10+,11-,12-/m0/s1 |
| InChI Key | QURSGHQPKUXLAD-MOBXTKCLSA-N |
| SMILES | CC1C(C(C(C(O1)OC2=C(C=C(C=C2)[N+](=O)[O-])Cl)O)O)O |
| Synonyms | 2-chloro-4-nitrophenyl alpha-L-fucopyranoside, 2-Cl-4-NP-F |
| Canonical SMILES | CC1C(C(C(C(O1)OC2=C(C=C(C=C2)[N+](=O)[O-])Cl)O)O)O |
| Isomeric SMILES | C[C@H]1[C@H]([C@H]([C@@H]([C@@H](O1)OC2=C(C=C(C=C2)[N+](=O)[O-])Cl)O)O)O |
CAS No: 157843-41-9 MDL No: MFCD04972056 Chemical Formula: C12H14ClNO7 Molecular Weight: 319.69 |
COA:
Name: 2-Chloro-4-nitrophenyl-alpha-L-fucopyranoside; CNP-AFU; CNPF
CAS:157843-41-9 M.F.: C12H14ClNO7 M.W.: 319.69
|
Items |
Standards |
Results |
|
Appearance |
White crystal powder |
Complies |
|
Solubility |
Soluble in DMF, insoluble in petroleum ether |
Complies |
|
Identification |
IR and HPLC |
Complies |
|
MS and NMR |
Should comply |
Complies |
|
Melting point |
150? ~ 160 ?C |
155? ~158 ? |
|
Specific rotation ( [?]20/D) |
-163o ~ -173o |
-172.5o |
|
Any other impurity |
Max. 0.5% |
Complies |
|
Heavy metal |
Max. 20ppm |
Complies |
|
Residue on ignition |
Max. 0.5% |
0.1% |
|
TLC |
Should be one spot |
One spot |
|
Assay by HPLC |
Min. 98% |
99.2% |
| Size | 1 G, 100 MG, 5 G, Other |
|---|
IUPAC:Fuc(a1-2)[GalNAc(a1-3)]Gal(b1-3)GlcNAc(b1-3)Gal(b1-4)b-Glc, G82858KU
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