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Our library includes a variety of enzymes derived from plants. Different enzymes will work with different functional groups on the substrate molecule. Some enzymes will add glucose. Others might add other sugars such as xylose, rhamnose, galalctose or glucuronic acid.
There are many examples of natural molecules that gain, lose or change properties when carbohydrates are attached. Hydroxylation, methylation, fluorination etc. are part of the everyday tool box for many chemists. Glycosylation is more unusual.
Glycosylation chemistry is difficult. Molecules with glycosylatable functional groups (-OH, -COOH, -NH, -SH) often need to have some of those groups blocked so that the sugar can be put where it is needed.
We take a natural approach.
Glycosyltransferase enzymes can glycosylate almost any molecule that has an appropriate side group. They can often work without any need to block side groups and many will work stereo-specifically.
By selecting the right enzyme we can also add multiple different sugars (glucose, galactose, xylose, glucuronic acid, rhamnose etc.).
We can also optimize (“evolve”) an enzyme to achieve a desired profile.
We’re happy to talk to you to see if glycosylation can work for your project. Get in touch by email info@glycodepot.com or use the request a quote form.
We can’t predict perfectly which enzymes will work with your molecule. But we can advise you on whether GLyco-Kit is likely to work for you.
Once we have found the enzyme(s) that makes the change that you need, we can provide its UGT sequences as well as the associated expression systems.
The enzymes are provided in four 96-well microtiter plates. Each well contains enough purified enzyme for about ten reactions. We can provide additional copies of each plate if you need them.
For confirmation of initial hits, quantification of activity and structure elucidation you will need HPLC or LC-MS analysis. Detailed protocols are provided with the kit. Online and phone support is available.
We can provide the identity and sequence of any of the enzymes along with an E. coli expression plasmid containing the encoding gene. We also offer related discovery and scale-up services
| No of Reaction | 1 Reaction, 3 Reaction, 5 Reaction |
|---|
| Name | Glycopeptidase; PNGaseF |
| Product Code | EN01022 |
| E.C. | 3.5.1.52 |
| Product Description | E. coli recombinant peptide-N4-(N-acetyl-β-glucosaminyl) asparagineamidase from Flavobacterium meningosepticum |
| Unit Definition | One unit is defined as the amount of enzyme that catalyzes the release of 1 nmol N-glycan from RNaseB per minute at 37 °C. |
10 in stock
| Name | Endo-β-N-acetylglucosaminidase A;Endo-A |
| Product Code | EN01023 |
| E.C. | 3.2.1.96 |
| Product Description | E. coli recombinant endo-β-N-acetylglucosaminidase from Arthrobacter protophormia |
| Unit Definition | One unit is defined as the amount of enzyme that catalyzes the release of 1 nmol N-glycan from RNaseB per minute at 37 °C. |
10 in stock
| Name | Galactokinase; BiGalK |
| Product Code | EN01018 |
| E.C. | 2.7.1.6 |
| Product Description | E. coli recombinant galactokinase from Bifidobacterium infantis |
| Unit Definition | One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol of Gal-1-P from galactose and ATP per minute at 37 °C. |
10 in stock
| Name | α2,8-sialyltransferase; Cstll |
| Catalog Number | EN01003 |
| E.C. | 2.4.99.8 |
| Product Description | E. coli recombinant α2,3/8-sialyltransferase from Campylobacter jejuni |
| Unit Definition | One unit is defined as the amount of enzyme that catalyzes the formation of 1 µmol Siaα2,3Lac from CMP-Sia and lactose per minute at 37 °C. |
10 in stock
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