Lacto-N-tetraose (LNT)

Lacto-N-tetraose (LNT) – Type 1 Chain HMO

Product Overview

Lacto-N-tetraose (LNT) is a naturally occurring human milk oligosaccharide (HMO) belonging to the lacto-series type-1 chain glycans. It is composed of four monosaccharide units — galactose (Gal), N-acetylglucosamine (GlcNAc), galactose (Gal), and glucose (Glc) — arranged in the linkage Galβ1-3GlcNAcβ1-3Galβ1-4Glc.

LNT is one of the most abundant neutral oligosaccharides in human breast milk and serves as a biologically active soluble glycan. It plays a significant role in shaping the infant gut microbiota and in mediating host–microbe interactions. Due to its well-defined structure, LNT is widely used as a model compound in studies involving microbiome modulation, pathogen adhesion inhibition, and carbohydrate recognition.

Applications

• Human milk oligosaccharide (HMO) and infant nutrition research
• Microbiome and probiotic growth studies (e.g., Bifidobacteria utilization)
• Pathogen binding and anti-adhesion assays
• Glycan-protein and lectin interaction analysis
• Analytical reference standard for HPLC, LC-MS, and NMR
• Glycosyltransferase and enzymatic synthesis studies
• Development of glycan microarrays and carbohydrate libraries

Key Features

• Naturally occurring HMO (type-1 chain)
• Structurally defined neutral oligosaccharide
• Biologically relevant for host–microbiota interaction studies
• High purity and reproducible performance
• Suitable for biochemical and analytical assays

Storage & Handling

• Store at −20 °C in a dry, sealed container
• Protect from moisture and repeated freeze-thaw cycles
• Bring vial to room temperature before opening