Lacto-N-triose II (LNT II)

Lacto-N-triose II (LNT II)

Product Description

Lacto-N-triose II (LNT II) is an important intermediate human milk oligosaccharide (HMO) building block consisting of GlcNAcβ1-3Galβ1-4Glc. It is the enzymatic hydrolysis product of Lacto-N-tetraose (LNT) and functions as a fundamental core structure in the biosynthesis of numerous complex HMOs including fucosylated and sialylated oligosaccharides.

In glycobiology, LNT II serves as a key substrate for glycosyltransferases such as β1-3-galactosyltransferase and fucosyltransferases, making it highly valuable for chemoenzymatic synthesis of HMOs and glycan libraries. The molecule also mimics host epithelial glycans and is used to study carbohydrate-binding proteins (lectins), bacterial adhesion mechanisms, and host-microbe interactions.

Because it represents a minimal HMO motif recognized by beneficial gut bacteria, LNT II is widely applied in microbiome research and probiotic metabolism studies, particularly for Bifidobacterium species capable of utilizing GlcNAc-containing oligosaccharides.

Key Features

• Core HMO trisaccharide intermediate
• Precursor for complex human milk oligosaccharides
• Enzyme substrate for glycosyltransferase reactions
• Useful for glycan engineering and synthesis studies
• Supports microbiota utilization research

Appearance & Solubility

• White to off-white amorphous powder
• Highly soluble in water
• Hygroscopic; readily forms clear solutions

Applications

• Chemoenzymatic synthesis of HMOs
• Glycosyltransferase and fucosyltransferase assays
• Lectin and receptor binding studies
• Host–pathogen interaction research
• Microbiome metabolism and probiotic utilization studies
• Analytical reference standard (HPLC, LC-MS, NMR)

Quality & Storage

• Research grade material
• Store at −20°C in a tightly sealed container
• Keep dry and protected from moisture
• Long-term stable when stored desiccated