n-Decyl-β-D-maltoside

n-Decyl-β-D-maltoside

n-Decyl-β-D-maltoside (CAS No. 82494-09-5) is a non-ionic surfactant and glycoside widely used in biochemical and biophysical research. It is particularly known for its applications in membrane protein studies due to its ability to solubilize and stabilize proteins without denaturing them.

Chemical and Physical Properties

• CAS Number: 82494-09-5
• MDL Number: MFCD00061624
• EINECS: Not specified
• Molecular Formula: C₂₂H₄₂O₁₁
• Molecular Weight: 482.56 g/mol
• Boiling Point: Not specified
• Melting Point: Not specified
• Flash Point: Not specified
• Density: 1.32 ± 0.1 g/cm³ at 20 °C (760 Torr)
• Appearance: Off-white powder

Structure and Composition

n-Decyl-β-D-maltoside consists of a decyl group attached to the anomeric carbon of maltose, forming a glycosidic bond. This structure imparts amphiphilic properties, making it effective as a detergent.

Biological Activity and Applications

1. Membrane Protein Research: n-Decyl-β-D-maltoside is extensively used for solubilizing membrane proteins, facilitating their purification and characterization in structural biology studies.
2. Stabilization of Enzymes: It helps in the stabilization and activation of various enzymes, making it valuable in biochemical assays.
3. Detection of Protein-Lipid Interactions: The compound is utilized to study interactions between proteins and lipids, contributing to the understanding of membrane dynamics.
4. Non-denaturing Properties: Its mild nature allows it to be used in studies involving protein-anesthetic interactions without altering protein structure.

Synthesis Methods

n-Decyl-β-D-maltoside can be synthesized through the glycosylation of maltose with decanol in the presence of acid catalysts or by using enzymatic methods that promote the formation of glycosidic bonds.

Safety and Handling

While generally considered safe for laboratory use, standard safety precautions should be observed when handling n-Decyl-β-D-maltoside to avoid skin and eye contact.

Storage Recommendations

For long-term stability, n-Decyl-β-D-maltoside should be stored at temperatures between 2–8°C in a cool, dry place away from light.

In summary, n-Decyl-β-D-maltoside is a versatile non-ionic detergent essential for membrane protein research and various biochemical applications, providing a means to study complex biological systems without compromising protein integrity.

Citations:

1. https://www.sigmaaldrich.com/IN/en/product/sigma/d4641
2. https://www.chemicalbook.com/ChemicalProductProperty_EN_CB8191322.htm
3. https://en.wikipedia.org/wiki/Maltoside
4. https://www.dojindo.com/ASIA/products/D382/
5. https://pubchem.ncbi.nlm.nih.gov/compound/5288728
6. https://pubchem.ncbi.nlm.nih.gov/compound/114880
7. https://avantiresearch.com/product/850521
8. https://www.caymanchem.com/product/25704/n-decyl-%CE%B2-d-maltoside