Peptide-N4-(N-acetyl-β-glucosaminyl) asparagineamidase (PNGaseF)

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Name	Glycopeptidase; PNGaseF
Product Code	EN01022
E.C.	3.5.1.52
Product Description	E. coli recombinant peptide-N4-(N-acetyl-β-glucosaminyl) asparagineamidase from Flavobacterium meningosepticum
Unit Definition	One unit is defined as the amount of enzyme that catalyzes the release of 1 nmol N-glycan from RNaseB per minute at 37 °C.

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Accepted Name
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase.
Alternative Name(s)
Glycopeptidase.
Glycopeptide N-glycosidase.
N-glycanase.
N-oligosaccharide glycopeptidase.
Reaction catalysed
Hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue
Comment(s)
Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino-acid residues in the substrate (cf. EC 3.5.1.26). Formerly EC 3.2.2.18.

2. MSDS

3. Tech Data Sheets/Manuals

Size	100U, 250U, 500U, Other

SKU: EN01016 Categories: Enzymes, Glycoenzymes Tags: Custom Products, Enzymes, Glycoenzymes

Name	β1,4-galactosyltransferase; LgtB
Catalog Number	EN01005
E.C.	2.4.1.90
Product Description	E. coli recombinant β1,4-galactosyltransferase from Neisseria meningitides
Unit Definition	One unit is defined as the amount of enzyme that catalyzes the formation of 1 µmol of Galβ1,4GlcNAc from UDP-Gal and GlcNAc per min at 37 °C.

Name	α1,4-galactosyltransferase; LgtC
Catalog Number	EN01006
E.C.	2.4.1.228
Product Description	E. coli recombinant α1,4-galactosyltransferase from Neisseria meningitides
Unit Definition	One unit is defined as the amount of enzyme that catalyzes the formation of 1 µmol of Galα1,4Lac from UDP-Gal and Lactose per minute at 37 °C.