RSL from Ralstonia solanacearum linked to Fluorescein

RSL from Ralstonia solanacearum linked to Fluorescein

RSL (Ralstonia solanacearum Lectin) linked to Fluorescein is a fluorescently labeled lectin used to study carbohydrate-mediated interactions in plant-pathogen systems. Derived from the soil-borne pathogen Ralstonia solanacearum, which causes bacterial wilt in crops like tomatoes and potatoes, this lectin plays a role in bacterial adhesion and virulence.

Key Characteristics of RSL:

• Function:
  RSL is a 9.9 kDa monomeric lectin related to fungal lectins, with affinity for fucose and other glycans. Unlike its homolog RS-IIL, which prefers mannose, RSL’s binding profile suggests a role in early host recognition, though its exact biological function in pathogenesis remains under investigation.
• Structural Features:

• • Shares structural motifs with fungal lectins but lacks the calcium-dependent binding seen in RS-IIL.
  • Forms stable complexes with fucosylated glycans, aiding in bacterial adhesion to plant surfaces.

• Fluorescein Conjugation:
  The fluorescein tag enables visualization of carbohydrate-binding activity in applications such as:

• • Fluorescence microscopy to map lectin distribution during plant infection.
  • Competitive binding assays to screen glycan-based inhibitors.
  • Analysis of biofilm matrix components in R. solanacearum.

Applications in Research:

1. Plant-Pathogen Interaction Studies:
   Tracks RSL’s binding to plant cell surface glycans, elucidating mechanisms of bacterial adhesion and colonization.
2. Biofilm Dynamics:
   Visualizes lectin-mediated stabilization of extracellular polysaccharides (EPS) in biofilms, critical for bacterial persistence.
3. Antimicrobial Development:
   Facilitates high-throughput screening of anti-adhesion compounds targeting RSL’s glycan-binding sites.

Production and Validation:

• Recombinantly expressed and purified for consistency.
• Functional integrity post-labeling confirmed via glycan-binding assays.

This tool advances research into R. solanacearum’s virulence strategies and supports efforts to develop targeted crop protection agents.

Citations:

1. https://www.evitachem.com/product/evt-1507402
2. https://pubmed.ncbi.nlm.nih.gov/15101976/
3. https://pmc.ncbi.nlm.nih.gov/articles/PMC6638453/
4. https://www.rcsb.org/structure/1UQX
5. https://www.rcsb.org/structure/2CHH
6. https://www.zeiss.com/meditec/en/myzeiss/peer-insights/articles/neurosurgery/advances-in-fluorescein-guided-neurosurgery.html
7. https://en.wikipedia.org/wiki/Fluorescein_(medical_use)
8. https://www.elicityl-oligotech.com/fluorescein/4667-4667.html
9. https://pmc.ncbi.nlm.nih.gov/articles/PMC92083/
10. https://en.wikipedia.org/wiki/Ralstonia_solanacearum