4-Nitrophenyl a-D-mannopyranoside
4-Nitrophenyl a-D-mannopyranoside is a chromogenic pNP enzyme substrate that enables the detection and quantification of a-D-mannosidase activity in various biological samples through a colorimetric assay. The hydrolysis of the substrate by the enzyme releases 4-nitrophenol, a yellow compound whose absorption can be measured spectrophotometrically. Applications for this substrate span biomedical research, disease diagnostics, and industrial enzyme production.
CAS No: 10357-27-4
Synonyms: Man-a-PNP
MDL No: MFCD00066002
Chemical Formula: C12H15NO8
Molecular Weight: 301.25 |
In Stock |
CAS Number |
10357-27-4 |
Product Name |
4-Nitrophenyl a-D-mannopyranoside |
IUPAC Name |
(2R,3S,4S,5S,6R)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol |
Molecular Formula |
C12H15NO8 |
Molecular Weight |
301.25 g/mol |
InChI |
InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11+,12+/m1/s1 |
InChI Key |
IFBHRQDFSNCLOZ-GCHJQGSQSA-N |
SMILES |
C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O |
Synonyms |
4′-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, alpha-PNM, p-nitrophenyl-alpha-mannoside, para-nitrophenyl-alpha-D-mannopyranoside, PNPAM |
Canonical SMILES |
C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O |
Isomeric SMILES |
C1=CC(=CC=C1[N+](=O)[O-])O[C@@H]2[C@H]([C@H]([C@@H]([C@H](O2)CO)O)O)O |
COA:
Name: 4-Nitrophenyl alpha-D-mannopyranoside
CAS: 10357-27-4 M.F.: C12H15NO8 M.W.: 301.25
Items
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Standards
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Results
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Appearance
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White crystalline power
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Positive
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Solubility
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Soluble in water and
insoluble in ether
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Positive
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NMR and MS
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Should comply
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Complies
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Identification
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IR and TLC
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Positive
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M.P.
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180? -185?
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180? -184?
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[a]D [c=0.44 inH2O].
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+150o – +160o
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+155.2o
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Loss Weight On Dryness
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Max. 0.5%
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Complies
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Beta-Isomer
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Max. 0.5%
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0.4%
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TLC (15%H2SO4-C2H5OH)
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One spot
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Complies
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Assay (HPLC)
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Min. 98%
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99.6%
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