Heparinase I, for EP Method

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OriginFlavobacterium Heparinum
CAS Number9025-39-2
EC Number4.2.2.7
Molecular Weight42.8 kDa
Purity (HPLC)≥ 99%
Specific Activity≥ 400 IU/mg (with heparin)
Concentration10 IU/mL
Storage Temperature -20 °C
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Heparinase I (heparin lyase I) cleaves heparin and to a lesser extent heparan sulfate (ratio approx. 3:1).

We provides heparinase I for determining 1,6-anhydro derivatives in heparin sodium. This enzyme has proven to meet the system suitability required by the European Pharmacopoeia method.

System suitability required by the European Pharmacopoeia Method:

– peak area ratio: maximum 1.15 for the peaks due to 1,6-anhydro ΔIS-IS and 1,6-anhydro ΔIS in the chromatogram obtained with reference solution (b);

– maximum 0.02 for the peaks due to ΔIS and reduced ΔIS in the chromatogram obtained with reference solution (c);

– resolution: minimum 1.5 between the peaks due to reduced ΔIA and 1,6-anhydro ΔIS in the chromatogram obtained with refer-ence solution (c);

– the content of 1,6-anhydro derivatives in heparin sodium CRS is within 1.5 per cent of the assigned content.

Applications:

Heparinase I is used for determining 1,6-anhydro derivatives in heparin sodium.

2. MSDS

3. Tech Data Sheets/Manuals

Size

0.1 IU, 0.5 IU, 1 IU

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