Heparinase III, for EP Method

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OriginFlavobacterium Heparinum
CAS Number37290-86-1
EC Number4.2.2.8
Molecular Weight70.8 kDa
Purity (HPLC)≥ 99%
Specific Activity≥200 IU/mg (with heparan sulfate)
Concentration5 IU/mL
Storage Temperature-20 °C
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Heparinase III (heparin lyase III) cleaves heparan sulfate exclusively, and does not cleave unfractionated heparin or low molecular weight heparins.

We provides heparinase III for determining 1,6-anhydro derivatives in heparin sodium. This enzyme has proven to meet the system suitability required by the European Pharmacopoeia method.

System suitability required by the European Pharmacopoeia Method:

– peak area ratio: maximum 1.15 for the peaks due to 1,6-anhydro ΔIS-IS and 1,6-anhydro ΔIS in the chromatogram obtained with reference solution (b);

– maximum 0.02 for the peaks due to ΔIS and reduced ΔIS in the chromatogram obtained with reference solution (c);

– resolution: minimum 1.5 between the peaks due to reduced ΔIA and 1,6-anhydro ΔIS in the chromatogram obtained with refer-ence solution (c);

– the content of 1,6-anhydro derivatives in heparin sodium CRS is within 1.5 percent of the assigned content.

Applications:

Heparinase III is used for determining 1,6-anhydro derivatives in heparin sodiu

2. MSDS

3. Tech Data Sheets/Manuals

Size

0.1 IU, 0.5 IU, 1 IU

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