From: $990.00
Size | 14 Sample Kit, 28 Sample Kit, 56 Sample Kit |
---|---|
Quantitative/Semi-Quantitative | Semi-Quantitative |
Number of Targets Detected | 70 |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Solid Support | Glass Slide |
Method Of Detection | Fluorescence Laser Scanner |
Design Principle | Sandwich-based, Label-based |
Research Area | Post-Translational Modifications, Glycosylation |
Shipping Type | Blue ice |
See Image/sIn this application, the Lectin Array 95 was used to detect specific glycosylations of purified Horseradish Peroxidase (HRP). Lectins BANLEC, BC2L-A, CALSEPA, GNA, HHA, NPA, PA-IIL, and PALa showed strong signals after incubation with 3.3 ug/mL Biotin-HRP followed by detection with streptavidin-fluorescence-dye (Figures A, B and C). The fluorescence signals from BANLEC, BC2L-A, CALSEPA, GNA, HHA, NPA, PA-IIL, and PALa were blocked in a concentration-dependent manner by HRP itself (Figures A and C), indicating that the signals were generated by lectin-HRP binding. These eight lectins are known to exhibit specific binding to mannose, which indicates that HRP contains mannose. After adding increasing amounts of mannose, the signals from BANLEC, BC2L-A, CALSEPA, GNA, HHA, NPA, PA-IIL, and PALa were reduced (Figures A and B).The reduction in signals from increasing concentrations of mannose confirms that HRP protein contains mannose in its glycocalyx. Additionally, the two lectins AAL and RS-FUC (fucose binding specificity) also showed strong interaction with HRP, which indicates the fucosylation of HRP. Overall, the results of the Lectin Array 95 were consistent with published literature regarding HRP glycosylation.
See Image/sUsing the lectin 95 array, we can discover the different glycoprotein profiles of serum samples, cell lysates, or purified glycoproteins. The images above show the profiles of the glycans from different types of samples including human serum, recombinant glycoproteins human HE4 and AFP, mouse TFF2, purified human IgG, and bacterial DE3 cell lysates detected by Biotin labeling and fluorescent dye-streptavidin.
Quantitative analysis of lectin-glycoprotein interactions. Example: a concentration series of glycoproteins detected with the lectin array could reveal concentration dependent effects of lectin-glycan binding.
Determine the profile of bacterial cell-surface glycans. Example: Cell lysate from bacteria can be Biotinylated and hybridized to the lectin array. Analysis of the binding pattern and correlation with the known carbohydrate-binding specificities of the lectins can determine the glycans on the cell membrane.
sample size | 14 Sample, 28 Sample, 56 Sample |
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Size | 4 Sample Kit, 8 Sample Kit |
---|---|
Species | Human |
Quantitative/Semi-Quantitative | Semi-Quantitative |
Number of Targets Detected | 500 |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Solid Support | Glass Slide |
Method Of Detection | Fluorescence Laser Scanner |
Design Principle | Sandwich-based |
Research Area | Post-Translational Modifications, Glycosylation |
Estimated Lead Time | 1-2 business days |
Shipping Type | Blue ice |
Storage | -20°C |
Size | 4 Sample Kit, 8 Sample Kit |
---|---|
Quantitative/Semi-Quantitative | Semi-Quantitative |
Number of Targets Detected | 507 |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Solid Support | Glass Slide |
Method Of Detection | Fluorescence Laser Scanner |
Design Principle | Sandwich-based |
Research Area | Post-Translational Modifications, Glycosylation |
Estimated Lead Time | 1-2 business days |
Shipping Type | Blue ice |
Storage | -20°C |
Size | 12 Sample Kit, 24 Sample Kit, 48 Sample Kit |
---|---|
Quantitative/Semi-Quantitative | Semi-Quantitative |
Number of Targets Detected | 95 |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Solid Support | Glass Slide |
Method Of Detection | Fluorescence Laser Scanner |
Design Principle | Sandwich-based, Label-based |
Research Area | Post-Translational Modifications, Glycosylation |
Estimated Lead Time | 1-2 business days |
Shipping Type | Blue ice |
Storage | -20°C |
Size | 4 Sample Kit, 8 Sample Kit |
---|---|
Species | Human |
Quantitative/Semi-Quantitative | Semi-Quantitative |
Number of Targets Detected | 1000 |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Solid Support | Glass Slide |
Method Of Detection | Fluorescence Laser Scanner |
Design Principle | Sandwich-based |
Research Area | Post-Translational Modifications, Glycosylation |
Estimated Lead Time | 1-2 business days |
Shipping Type | Blue ice |
Storage | -20°C |
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