Azido-modified analogue of UDP-GlcNAc; serves as a substrate for O-GlcNAc transferase (OGT) and N-acetylglucosaminyltransferases enabling metabolic and chemoenzymatic glycan labeling; the azidoacetyl handle permits bioorthogonal conjugation via copper-catalyzed azide–alkyne cycloaddition (CuAAC) and strain-promoted azide–alkyne cycloaddition (SPAAC); can be epimerized to UDP-GalNAz by UDP-galactose-4-epimerase (GALE); widely used in O-GlcNAc proteomics, glycan imaging, and selective probing of intracellular O-GlcNAcylation
Uridine-5’-diphospho-6-azido-6-deoxy-D-glucose, Disodium Salt
Synonyms
UDP-6-N3-Glc·2Na, UDP-α-6N3-Glucose Disodium Salt, UDP-6-Azido-6-deoxy-D-Glucose Disodium Salt
Molecular Formula
C15H21N5O16P2 · 2Na
Molecular Weight
635.28 g/mol (disodium salt)
CAS No.
537039-67-1
Appearance
White to off-white lyophilized powder
Purity
≥95% (HPLC)
Solubility
Soluble in water
Storage
−20°C, protect from moisture and light
Application
Azido-modified nucleotide sugar donor substrate for glucosyltransferases; the C6-azido handle enables bioorthogonal detection via copper-catalyzed azide–alkyne cycloaddition (CuAAC) and strain-promoted azide–alkyne cycloaddition (SPAAC); used for selective labeling of 5-hydroxymethylcytosine (5-hmC) in genomic DNA, glycan imaging, and glycoproteomic profiling