X-Gluc Dicyclohexylamine

X-Gluc Dicyclohexylamine

X-Gluc Dicyclohexylamine (CAS No. 18656-96-7) is a chemical compound widely utilized in molecular biology as a reagent for detecting β-glucuronidase, an enzyme produced by Escherichia coli. This compound is particularly important in the GUS (β-glucuronidase) reporter system, which enables researchers to mark and select the expression of target genes. The ability to visualize gene expression through colorimetric assays makes X-Gluc Dicyclohexylamine a valuable tool in genetic studies and various experimental applications.

Chemical Properties

• CAS Number: 18656-96-7
• MDL Number: MFCD28016036
• EINECS: 239-603-0
• Molecular Formula: C14_{14}14H14_{14}14NO7_{7}7Br.C6_{6}6H13_{13}13N
• Molecular Weight: 603.93 g/mol
• Boiling Point: 742 °C at 760 mmHg
• Melting Point: 249 °C
• Solubility: Soluble in DMSO (≥ 30 mg/mL) and DMF (2% solution)
• Flash Point: Not available

Structure and Composition

X-Gluc Dicyclohexylamine consists of two main components:

1. X-Gluc Component: This part serves as a substrate for β-glucuronidase, undergoing enzymatic cleavage to produce a blue precipitate that can be easily visualized.
2. Dicyclohexylamine Component: This enhances the solubility and stability of the compound in various solvents, facilitating its use in laboratory applications.

The molecular formula indicates the presence of bromine (Br), nitrogen (N), and multiple hydroxyl (-OH) groups, which are essential for its reactivity as a chromogenic substrate.

Applications

X-Gluc Dicyclohexylamine is primarily used in:

Molecular Biology

• Gene Expression Studies: It acts as a substrate for β-glucuronidase, allowing researchers to visualize and quantify gene expression through colorimetric assays.

Histochemical Staining

• X-Gluc can be applied to tissue samples for histochemical studies, enabling researchers to map gene expression patterns in various biological specimens.

Colorimetric Assays

• The compound facilitates colorimetric assays where enzyme activity can be quantified based on the intensity of the blue color produced during the reaction.

Safety and Handling

While X-Gluc Dicyclohexylamine is not classified as hazardous under standard conditions, it is essential to follow appropriate safety protocols:

1. Personal Protective Equipment (PPE): Always wear gloves, goggles, and lab coats when handling this chemical.
2. Ventilation: Work in a well-ventilated area or fume hood to avoid inhalation of vapors.
3. Storage Conditions: Store at -20 °C in a dry environment away from light and moisture to maintain stability.
4. Disposal Guidelines: Dispose of waste according to local regulations for hazardous materials.

Summary

In summary, X-Gluc Dicyclohexylamine (CAS No. 18656-96-7) is an essential reagent in molecular biology for detecting β-glucuronidase activity. Its ability to produce a visible blue precipitate upon enzymatic cleavage makes it invaluable for gene expression studies and enzymatic assays. Proper storage and handling are crucial to ensure its efficacy for experimental applications.

Citations:

1. https://www.chemsrc.com/en/cas/18656-96-7_441835.html
2. https://static.cymitquimica.com/products/IN/pdf/sds-DA003MJ3.pdf
3. https://www.glpbio.com/kr/x-gluc-dicyclohexylamine.html
4. https://www.adooq.com/x-gluc-dicyclohexylamine.html
5. https://en.wikipedia.org/wiki/X-Gluc
6. https://www.clinisciences.com/en/other-products-186/x-gluc-dicyclohexylamine-18656-231007815.html
7. https://www.clinisciences.com/en/other-products-186/x-gluc-dicyclohexylamine-18656-231005843.html
8. https://www.medchemexpress.com/x-gluc-cyclohexanamine.html