2-Nitrophenyl-beta-D-galactopyranoside

2-Nitrophenyl b-D-galactopyranoside - non-animal origin o-Nitrophenyl ?-D-galactopyranoside (ONPG) is a chromogenic substrate for ?-galactosidase. Upon enzymatic cleavage by ?-galactosidase, o-nitrophenol is released, which can be quantified by colorimetric detection at 420 nm as a measure of ?-galactosidase activity. 2-nitrophenyl beta-D-galactoside is a beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position. It has a role as a chromogenic compound. It is a beta-D-galactoside and a C-nitro compound. It derives from a 2-nitrophenol. CAS Number 369-07-3 Product Name 2-Nitrophenyl-beta-D-galactopyranoside IUPAC Name (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol Molecular Formula C??H??NO? Molecular Weight 301.25 g/mol InChI InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1 InChI Key KUWPCJHYPSUOFW-UHFFFAOYSA-N SMILES C1=CC=C(C(=C1)[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O Solubility 45.2 [ug/mL] Synonyms 2-nitrophenyl-alpha-D-galactoside, 2-nitrophenylgalactoside, 2-nitrophenylgalactoside, (alpha-D)-isomer, 2-nitrophenylgalactoside, (beta-D)-isomer, o-nitrophenyl-beta-D-galactopyranoside, ONPG, ONPG cpd, ortho-nitrophenyl-beta-D-galactoside Canonical SMILES C1=CC=C(C(=C1)[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O Isomeric SMILES C1=CC=C(C(=C1)[N+](=O)[O-])O[C@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O CAS No: 369-07-3 Synonyms: ONPG MDL No: MFCD00063255 Chemical Formula: C12H15NO8 Molecular Weight: 301.25 COA: Product name : 2-Nitrophenyl-?-D-galactopyranoside CAS: 369-07-3 M.F. : C 12 H 15 O 8 M .W. : 301.25 Items Standards Results Appearance White or off-white crystal powder Complies Solubility Soluble in DMF or hot and insoluble in ether Complies Color and Turbidity ? in 15mg/ml in water ? Colorless or light yellow and clear Comply NMR and MS Should comply Comply Specific rotation [?]20/D, in water -68 ~ -72? -70? Melting point 186-193 ?C (Dec.) 188-190 ? Carbon 46.9 - 48.8 % Complies Nitrogen 4.5 - 4.8 % Complies Loss weight on dryness Max. 0.5% 0.11% Residue on ignition 0.5% max 0.2% Heavy metals Max. 20 ppm Complies Assay by HPLC Min. 98% 99.2% Purity (Enzymatic) Min. 98% Complies References: 1. Maruhn D, Clin. Chim. Acta 1976, 73, 453 2. Levvy GA, Conchie J, Methods Enzymol. 1966, 8, 571-84 3. Naider F, et al., Biochemistry 1972, 11, 3202-7