4-Methylumbelliferyl-(beta)-D-glucuronidehydrate

4-Methylumbelliferyl bD-glucuronide dihydrate (MUG) 4-Methylumbelliferyl beta-D-glucuronide (MUG) is commonly used as a substrate for detecting beta;-glucuronidase. MUG is used for identifying E. coli contamination in food, drinkingwater, clinical samples and for rapid bacterial identification in blood cultures. It is recommended in international normatives (ISO, DIN) as a fluorogenic substrate for detection of E. coli. MUG is also a tool assaying the glucuronidase gene in plants. 4-Methylumbelliferyl-?-D-glucuronide hydrate is a fluorogenic substrat (?ex=362 nm, ?em=445 nm). CAS Number 881005-91-0 Product Name 4-Methylumbelliferyl-beta-D-glucuronide hydrate IUPAC Name (2S,3S,4S,5R,6S)-3,4,5-trihydroxy-6-(4-methyl-2-oxochromen-7-yl)oxyoxane-2-carboxylic acid;hydrate Molecular Formula C??H??O?? Molecular Weight 370.31 g/mol ikB InChI=1S/C16H16O9.H2O/c1-6-4-10(17)24-9-5-7(2-3-8(6)9)23-16-13(20)11(18)12( 19)14(25-16)15(21)22;/h2-5,11-14,16,18-20H,1H3,(H,21,22);1H2/t11-,12-,13+, 14-,16+;/m0./s1 InChI Key URVSQZMOFUEQAW-YYHOVTOASA-N SMILES CC1=CC(=O)OC2=C1C=CC(=C2)OC3C(C(C(C(O3)C(=O)O)O)O)OO Canonical SMILES CC1=CC(=O)OC2=C1C=CC(=C2)OC3C(C(C(C(O3)C(=O)O)O)O)OO Isometric SMILES CC1=CC(=O)OC2=C1C=CC(=C2)O[C@H]3[C@@H]([C@H]([C@@H]([C@H](O3 )C(=O)O)O)O)OO CAS No: 881005-91-0,6160-80-1 Synonyms: MUG4-Methylumbelliferyl bD-Glucopyranosiduronic acid MDL No: MFCD00151678 Chemical Formula: C16H16O9?2H2O Molecular Weight: 388.32 COA: Product name : 4-Methylumbelliferyl beta-D-glucuronide dihydrate CAS: 881005-91-0 MF : C 16 H 16 O 9 .2H 2 O M.W.: 352.3 (anhydrous) Batch No : 20130212 Quantity .: 12.6g Items Standards Results Appearance white crystal powder Complies Solubility Soluble in hot DMF, insoluble in ether Complies Identification IR and HPLC Complies Appearance of solution Dissolve0.5gin 10mL of DMF, and the solution should be clear Complies Specific rotation ([?] 22/D , c =0.25 inH 2 O) -100 o ~ -110 o -105.5o ? Water Content Max.15% 10.5% Any other impurity Max. 0.5% Complies Heavy metal Max. 20ppm Complies Residue on ignition Max. 0.5% 0.1% 4-Methylumbelliferone Max. 50ppm Complies Assay by HPLC Min. 98% 99.2%