4-Nitrophenyl-beta-D-glucopyranoside

4-Nitrophenyl b-D-glucopyranoside 4-Nitrophenyl-?-D- glucopyranoside is a chromogenic ?-D-glucosidase substrate, producing a yellow solution upon cleavage. 4-Nitrophenyl ?-D-glucopyranoside is a chromogenic substrate for ?-glucosidase. Upon hydrolysis by ?-glucosidase, 4-nitrophenol is released, which can be quantified by colorimetric detection at 405 nm as a measure of ?-glucosidase activity. 4-nitrophenyl beta-D-glucoside is a beta-D-glucoside that is beta-D-glucopyranose in which the anomeric hydroxy hydrogen is replaced by a 4-nitrophenyl group. It has a role as a chromogenic compound. It is a beta-D-glucoside and a C-nitro compound. It derives from a 4-nitrophenol. CAS Number 2492-87-7 Product Name 4-Nitrophenyl beta-D-glucopyranoside IUPAC Name (2R,3S,4S,5R,6S)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol Molecular Formula C??H??NO? Molecular Weight 301.25 g/mol InChI InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11-,12-/m1/s1 InChI Key IFBHRQDFSNCLOZ-RMPHRYRLSA-N SMILES C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O Synonyms 4-nitrophenyl beta-D-glucoside, 4-nitrophenyl beta-D-glucoside hydride, 4-nitrophenyl beta-D-glucoside ion (1-), 4-nitrophenylglucoside, p-nitrophenyl-beta-D-glucopyranoside, para-nitrophenyl beta-D-glucoside, PNP-beta-Glu Canonical SMILES C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O Isomeric SMILES C1=CC(=CC=C1[N+](=O)[O-])O[C@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CO)O)O)O CAS No: 2492-87-7 Synonyms: PNP-glucoside MDL No: MFCD00006593 Chemical Formula: C12H15NO8 Molecular Weight: 301.25 In Stock,??????,??????. COA: Product name : 4'-Nitrophenyl-beta-D-glucopyranoside CAS: 2492-87-7 M.F. : C 12 H 15 NO 8 M .W. : 301.25 Items Standards Results Appearance White crystal powder Complies Solubility Soluble in hot water, insoluble in ether Complies Identification IR and HPLC Complies Melting point 165 ? ~ 168 ?C 165 ? ~167 ? Specific rotation ( [?] 20/D , in H 2 O) -102 o ~ -105 o -103.5 o Any other impurity Max. 0.5% Complies Total impurity Max. 2% Complies Heavy metal Max. 20ppm Complies Residue on ignition Max. 0.5% 0.1% TLC One spot One spot Assay by HPLC Min. 98% 99.5%