4-Nitrophenyla-D-mannopyranoside

4-Nitrophenyl a-D-mannopyranoside 4-Nitrophenyl a-D-mannopyranoside is a chromogenic pNP enzyme substrate that enables the detection and quantification of a-D-mannosidase activity in various biological samples through a colorimetric assay. The hydrolysis of the substrate by the enzyme releases 4-nitrophenol, a yellow compound whose absorption can be measured spectrophotometrically. Applications for this substrate span biomedical research, disease diagnostics, and industrial enzyme production. CAS No: 10357-27-4 Synonyms: Man-a-PNP MDL No: MFCD00066002 Chemical Formula: C12H15NO8 Molecular Weight: 301.25 In Stock CAS Number 10357-27-4 Product Name 4-Nitrophenyl a-D-mannopyranoside IUPAC Name (2R,3S,4S,5S,6R)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol Molecular Formula C12H15NO8 Molecular Weight 301.25 g/mol InChI InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11+,12+/m1/s1 InChI Key IFBHRQDFSNCLOZ-GCHJQGSQSA-N SMILES C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O Synonyms 4'-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, alpha-PNM, p-nitrophenyl-alpha-mannoside, para-nitrophenyl-alpha-D-mannopyranoside, PNPAM Canonical SMILES C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O Isomeric SMILES C1=CC(=CC=C1[N+](=O)[O-])O[C@@H]2[C@H]([C@H]([C@@H]([C@H](O2)CO)O)O)O COA: Name : 4-Nitrophenyl alpha-D-mannopyranoside CAS : 10357-27-4 M.F. : C 12 H 15 NO 8 M .W. : 301.25 Items Standards Results Appearance White crystalline power Positive Solubility Soluble in water and insoluble in ether Positive NMR and MS Should comply Complies Identification IR and TLC Positive M.P. 180 ? -185 ? 180 ? -184 ? [a] D [c=0.44 inH 2 O]. +150 o - +160 o +155.2 o Loss Weight On Dryness Max. 0.5% Complies Beta-Isomer Max. 0.5% 0.4% TLC (15%H 2 SO 4 -C 2 H 5 OH) One spot Complies Assay (HPLC) Min. 98% 99.6%