4-Nitrophenylbeta-D-cellotrioside

4-Nitrophenyl b-D-cellotrioside 4-Nitrophenyl b-D-cellotrioside is a fluorogenic substrate that is used in enzyme assays, chromogenic substrates, and chemiluminescence. It is a high-purity chemical that can be used to stain cells or tissue culture media. 4-Nitrophenyl b-D-cellotrioside may be used for the detection of bacterial contamination in food, water, and other environmental samples. 4NPCT can also be conjugated with an antibody or antigen for use as a ligand in immunoassays. 4NPCT has been shown to be an excellent substrate for bacterial ?-glucuronidase activity and is useful for the detection of bacteria such as Proteus mirabilis, Pseudomonas aeruginosa, E. coli and S. typhimurium. 4-Nitrophenyl ?-D-cellotrioside is a chromogenic substrate for endoglucanases and cellobiohydrolases. Hydrolysis of this substrate by these enzymes liberates 4-nitrophenol, which generates a yellow color that is measured by monitoring absorbance at 405 nm. CAS Number 106927-48-4 Product Name 4-Nitrophenyl beta-D-cellotrioside IUPAC Name 2-[6-[4,5-dihydroxy-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Molecular Formula C24H35NO18 Molecular Weight 625.53g/mol InChI InChI=1S/C24H35NO18/c26-5-10-13(29)14(30)17(33)23(39-10)42-21-12(7-28)41-24(19(35)16(21)32)43-20-11(6-27)40-22(18(34)15(20)31)38-9-3-1-8(2-4-9)25(36)37/h1-4,10-24,26-35H,5-7H2 InChI Key BETIRLUWOMCBBJ-ZENQEEDISA-N SMILES C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)OC3C(C(C(C(O3)CO)OC4C(C(C(C(O4)CO)O)O)O)O)O)O)O Synonyms b-D-Glc[1-4]-b-D-Glc-[1-4]-b-D-Glc-1-OC6H4-p-NO2 Canonical SMILES C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)OC3C(C(C(C(O3)CO)OC4C(C(C(C(O4)CO)O)O)O)O)O)O)O CAS No: 106927-48-4 MDL No: MFCD00133737 Chemical Formula: C24H35NO18 Molecular Weight: 625.53