5-Bromo-4-chloro-3-indolyl-beta-D-glucuronidesodiumsalt

5-Bromo-4-chloro-3-indolyl b-D-glucuronide sodium salt trihydrate 5-Bromo-4-chloro-3-indoxyl-?-D-glucuronic acid sodium salt anhydrous is a bromoquinone that is highly specific for the ? -glucuronidase enzyme. The enzyme is expressed in plants, rhizogenes and family Enterobacteriaceae, but not in mitochondria or Staphylococcus aureus. 5-Bromo-4-chloro-3-indoxyl-? -D glucuronic acid sodium salt anhydrous has been used to detect ? -glucuronidase activity in plant cells, which can be measured by colorimetric techniques. It has also been used as a diagnostic aid to identify members of the family Enterobacteriaceae, including E. coli and Klebsiella pneumoniae, by using biochemical tests such as glucose oxidase and dehydrogenase activity on plates. CAS No: 370100-64-4,129541-41-9 Synonyms: X-Glucuronide trihydrateX-GlcA Na MDL No: MFCD01861962 Chemical Formula: C14H12BrClNNaO7?3H2O Molecular Weight: 498.64 CAS Number 129541-41-9 Product Name 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt IUPAC Name sodium;(2S,3S,4S,5R,6S)-6-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-3,4,5-trihydroxyoxane-2-carboxylate Molecular Formula C14H12BrClNNaO7 Molecular Weight 444.59 g/mol InChI InChI=1S/C14H13BrClNO7.Na/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;/h1-3,9-12,14,17-20H,(H,21,22);/q;+1/p-1/t9-,10-,11+,12-,14+;/m0./s1 InChI Key IBLSVGDGSKUDCT-CYRSAHDMSA-M SMILES C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)C(=O)[O-])O)O)O)Cl)Br.[Na+] Canonical SMILES C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)C(=O)[O-])O)O)O)Cl)Br.[Na+] Isomeric SMILES C1=CC(=C(C2=C1NC=C2O[C@H]3[C@@H]([C@H]([C@@H]([C@H](O3)C(=O)[O-])O)O)O)Cl)Br.[Na+] COA: Product name : 5-Bromo-4-chloro-3-indolyl b-D-glucuronide sodium salt CAS: 129541-41-9 M.F. : C 14 H 12 BrClNNaO 7 .XH 2 O M.W. : 498.64 Batch No : 20120425 Quantity : 10g Items Standards Results Appearance White to off-white powder Complies Solubility Soluble in hot water, insoluble in ether Complies NMR and MS Should comply Comply Identification IR and HPLC Complies Water (K.F.) Max. 10% 6.7% Heavy metal Max. 20ppm Complies Residue on ignition Max. 0.5% 0.1% TLC One spot One spot Assay by HPLC Min. 98% 98.3% *Substrate for assay of ?-glucuronidase activity.