beta-D-Xylopyranoside,2-nitrophenyl4-O-beta-D-xylopyranosyl-

2-Nitrophenyl b-D-xylobioside 2-Nitrophenyl b-D-xylobioside is a chromogenic substrate for the enzyme ?-galactosidase. It is used in food testing, diagnostics, and environmental testing as an indicator of microbial contamination. 2-Nitrophenyl b-D-xylobioside is also used in fluorescence assay for the determination of ?-galactosidase activity. In this assay, it is converted to a fluorescent product by ?-galactosidase and can be detected by a fluorometer. 2NBPX has been shown to be non toxic and biodegradable, with good solubility at pH 7.0 and a reasonably low melting point. 2NBPX is commonly used as a ligand for affinity chromatography or as an enzyme substrate for quantitative analysis of ?-galactosidase activity in biological samples. CAS Number 157956-98-4 Product Name 2-[4,5-Dihydroxy-6-(2-nitrophenoxy)oxan-3-yl]oxyoxane-3,4,5-triol IUPAC Name 2-[4,5-dihydroxy-6-(2-nitrophenoxy)oxan-3-yl]oxyoxane-3,4,5-triol Molecular Formula C??H??NO?? Molecular Weight 403.34 InChI InChI=1S/C16H21NO11/c18-8-5-25-15(13(21)11(8)19)28-10-6-26-16(14(22)12(10)20)27-9-4-2-1-3-7(9)17(23)24/h1-4,8,10-16,18-22H,5-6H2 SMILES C1C(C(C(C(O1)OC2COC(C(C2O)O)OC3=CC=CC=C3[N+](=O)[O-])O)O)O Synonyms o-NP-X; 2-Nitrophenyl 4-o-?-D-xylopyranosyl-?-D-xylopyranoside CAS No: 157956-98-4 Synonyms: ONP-xylobioside MDL No: MFCD01863440 Chemical Formula: C16H21NO11 Molecular Weight: 403.34 References: 1. Biosci. Biotechnol. Biochem. 1996, 60(6), 9832. Honda Y, Kitaoka M, Sakka K, Ohmiya K, Hayashi K, J. Biosci. Bioeng. 2002, Vol93, Pt3, p313-317