Luc-coliforms,Luc-beta-Gal

Luc-coliforms;6-O-(beta-D-Galactopyranosyl)luciferin; D-Luciferin-6-O-beta-D-galactopyranoside; Luc-beta-Gal Ultrasensitive chemiluminescent beta-galactosidase substrate. D-luciferin analog with beta-galactoside on 6-O position. Beta-galactoside must be hydrolysed by beta-galactosidase before luciferin can be acted on by firefire luciferase. Can detect as little as 3 x 10-19 mol of beta-galactosidase per assay. Luciferin-O-galactopyranoside is a bioluminogenic substrate for beta-galactosidase. Luc-coliforms are gram-negative bacteria that belong to the family Enterobacteriaceae. They can be found in water and soil, as well as on plants and mammalian tissue. Luc-coliforms produce a green light when they react with an antibody solution, which is used in immunoassays for the detection of this bacterium. This bacterial family includes Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Citrobacter freundii. These bacteria are specific to mammals and do not grow on plant tissue or clinical chemistry media. Luc-coliforms have been shown to produce enzyme activity that can reduce metal ions such as Fe3+, Cu2+, Zn2+, Ni2+, Co2+ and Mn2+. CAS Number 131474-38-9 Product Name Luciferin-O-galactopyranoside IUPAC Name (4S)-2-[6-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-1,3-benzothiazol-2-yl]-4,5-dihydro-1,3-thiazole-4-carboxylic acid Molecular Formula C17H18N2O8S2 Molecular Weight 442.5 g/mol InChI InChI=1S/C17H18N2O8S2/c20-4-9-11(21)12(22)13(23)17(27-9)26-6-1-2-7-10(3-6)29-15(18-7)14-19-8(5-28-14)16(24)25/h1-3,8-9,11-13,17,20-23H,4-5H2,(H,24,25)/t8-,9-,11+,12+,13-,17-/m1/s1 InChI Key OUKXHVUTHGDBNY-VEAFCXQSSA-N SMILES C1C(N=C(S1)C2=NC3=C(S2)C=C(C=C3)OC4C(C(C(C(O4)CO)O)O)O)C(=O)O Solubility Soluble in DMSO Synonyms 6-O-beta-D-galactopyranosyl-luciferin, D-luciferin-O-beta-galactopyranoside, luciferin-O-Gal, luciferin-O-galactopyranoside Canonical SMILES C1C(N=C(S1)C2=NC3=C(S2)C=C(C=C3)OC4C(C(C(C(O4)CO)O)O)O)C(=O)O Isomeric SMILES C1[C@@H](N=C(S1)C2=NC3=C(S2)C=C(C=C3)O[C@H]4[C@@H]([C@H]([C@H]([C@H](O4)CO)O)O)O)C(=O)O