RSL from Ralstonia solanacearum linked to Fluorescein

RSL from Ralstonia solanacearum linked to Fluorescein RSL (Ralstonia solanacearum Lectin) linked to Fluorescein is a fluorescently labeled lectin used to study carbohydrate-mediated interactions in plant-pathogen systems. Derived from the soil-borne pathogen Ralstonia solanacearum , which causes bacterial wilt in crops like tomatoes and potatoes, this lectin plays a role in bacterial adhesion and virulence. Key Characteristics of RSL: Function : RSL is a 9.9 kDa monomeric lectin related to fungal lectins, with affinity for fucose and other glycans. Unlike its homolog RS-IIL, which prefers mannose, RSL’s binding profile suggests a role in early host recognition, though its exact biological function in pathogenesis remains under investigation. Structural Features : Shares structural motifs with fungal lectins but lacks the calcium-dependent binding seen in RS-IIL. Forms stable complexes with fucosylated glycans, aiding in bacterial adhesion to plant surfaces. Fluorescein Conjugation : The fluorescein tag enables visualization of carbohydrate-binding activity in applications such as: Fluorescence microscopy to map lectin distribution during plant infection. Competitive binding assays to screen glycan-based inhibitors. Analysis of biofilm matrix components in R. solanacearum . Applications in Research: Plant-Pathogen Interaction Studies : Tracks RSL’s binding to plant cell surface glycans, elucidating mechanisms of bacterial adhesion and colonization. Biofilm Dynamics : Visualizes lectin-mediated stabilization of extracellular polysaccharides (EPS) in biofilms, critical for bacterial persistence. Antimicrobial Development : Facilitates high-throughput screening of anti-adhesion compounds targeting RSL’s glycan-binding sites. Production and Validation: Recombinantly expressed and purified for consistency. Functional integrity post-labeling confirmed via glycan-binding assays. This tool advances research into R. solanacearum ’s virulence strategies and supports efforts to develop targeted crop protection agents. Citations: https://www.evitachem.com/product/evt-1507402 https://pubmed.ncbi.nlm.nih.gov/15101976/ https://pmc.ncbi.nlm.nih.gov/articles/PMC6638453/ https://www.rcsb.org/structure/1UQX https://www.rcsb.org/structure/2CHH https://www.zeiss.com/meditec/en/myzeiss/peer-insights/articles/neurosurgery/advances-in-fluorescein-guided-neurosurgery.html https://en.wikipedia.org/wiki/Fluorescein_(medical_use) https://www.elicityl-oligotech.com/fluorescein/4667-4667.html https://pmc.ncbi.nlm.nih.gov/articles/PMC92083/ https://en.wikipedia.org/wiki/Ralstonia_solanacearum