Glycan Array 300

Introduction Glycocalyx, literally meaning ‘sugar coat’, is an extracellular polymeric coating surrounding many prokaryotic and eukaryotic cells consisting of glycoproteins, glycolipids, proteoglycans and glycosaminoglycans. The constituents of the glycocalyx play an important role in the process of protein folding and trafficking, cell signaling and intercellular interactions, cell pathogen interactions, and immunity. However, detection tools for the research of glycobiology are currently in very limited supply. Raybiotech has pioneered the development of antibody arrays which are now widely applied in the research community with thousands of peer reviewed publications, including in Cell and Nature. Taking advantage of advancements in microarray technology developed for antibody arrays, we have developed a new generation glycan array, the Glycan-300, soon after previously launching the Glycan-100. The Glycan-300 is currently the largest commercially available glycan array for screening protein-carbohydrate interactions. This array will help researchers: Identify glycan binding partners in biological samples Identify whether target proteins are carbohydrate binding proteins Probe binding of pathogens including bacteria, viruses and whole cells to glycans 1-3 Profile the substrate specificity of enzymes (glycosyltransferases, glycosidases, etc.) 4-6 Profile the inflammatory and/or immune response. The 300 synthetic glycans featured in the new generation Glycan Glycan-300 array consists of four groups of targets including the 100 glycans from the Raybio Glycan-100 array and an additional 80 N-glycans, 50 glycolipid glycans, and 50 human milk oligosaccharide glycans. The included glycans have been frequently identified as structures showing important binding functions in the literature. For example, a variety of sialosides have been shown to bind to the influenza virus with a serotype-specific pattern. 7-9  Galectins, which are involved in apoptosis, cell adhesion and T-cell activation suppression, function by binding beta-galactosides. 10-12  Glycosphingolipids interact with growth factor receptors to modify signal transduction or with bacterial toxin subunits, such as Vero(Shiga) toxin, cholera toxin, and the heat labile E.coli toxins, to induce cell apoptosis 13 . Additionally, oligosaccharides from human milk bind to microbes to promote growth of normal flora and inhibit pathogens in the gastrointestinal tract 14 . This array provides a powerful tool for researchers in glycobiology and other fields. Glycan Names: Download Full Glycan List  |  Download Glycan Structures N-Glycans Glycolipid Glycans Human Milk Oligosaccharides Tandem Epitopes Other Glycans USER MANUAL N-Glycans Man-α-1,6-(Man-α-1,3-)Man-α-1,6-(GlcNAc-β-1,2-Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Man-α-1,6-(Man-α-1,3-)Man-α-1,6-(Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Man-α-1,6-(Man-α-1,3-)Man-α-1,6-(Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-) Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Man-α-1,6-(Man-α-1,3-)Man-α-1,6-(Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-) Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Man-α-1,6-(Man-α-1,3-)Man-α-1,6-[Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,3-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Man-α-1,6-(Man-α-1,3-)Man-α-1,6-[Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2- Man-α-1,3-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Gc-α-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Man-α-1,6-(GlcNAc-β-1,2-Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 GlcNAc-β-1,2-Man-α-1,6-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,6-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,6-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 GlcNAc-β-1,2-Man-α-1,6-(Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-(Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-(Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-(Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,6-(Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,2-Man-α-1,6-(Man-α-1,3-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 GlcNAc-β-1,2-Man-α-1,6-[GlcNAc(3Ac)-β-1,2-Man-α-1,3-Man-β-1,4-GlcNAc-β-1,4-GlcNAc-Sp5 Glycolipid Glycans Neu5Ac-α-2,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,3-Gal-β-1,4-Glc-Sp5 Kdn-α-2,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-(GalNAc-b-1,4-)Gal-b-1,4-Glc-Sp5 Neu5Gc-a-2,3-(GalNAc-b-1,4-)Gal-b-1,4-Glc-Sp5 Kdn-a-2,3-(GalNAc-b-1,4-)Gal-b-1,4-Glc-Sp5 Neu5Ac-a-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-b-1,4-Glc-Sp5 Neu5Gc-a-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-b-1,4-Glc-Sp5 Kdn-a-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-b-1,4-Glc-Sp5 Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-Sp5 Kdn-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-Glc-Sp5 Kdn-α-2,8-Neu5Gc-α-2-,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,8-Kdn-α-2,3-Gal-β-1,4-Glc-Sp5 Kdn-α-2,8-Kdn-α-2,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,8-Neu5Ac-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Neu5Ac-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Kdn-α-2,8-Neu5Ac-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,8-Neu5Gc-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Neu5Gc-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Kdn-α-2,8-Neu5Gc-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Kdn-α-2,3-(GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,8-Neu5Ac-α-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Neu5Ac-α-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,8-Neu5Gc-α-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 Kdn-α-2,8-Neu5Gc-α-2,3-(Gal-b-1,3-GalNAc-b-1,4-)Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-a-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-a-1,4-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-a-1,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Kdn-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,8-Neu5Ac-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-a-2,8-Neu5Ac-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,8-Neu5Gc-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-a-2,8-Neu5Gc-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-a-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Kdn-a-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-a-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-a-1,4-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Kdn-a-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-a-2,3-Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Kdn-a-2,3-Gal-β-1,3-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-α-1,4-Gal-β-1,4-Glc-Sp5 GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Neu5Gc-a-2,3-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Kdn-a-2,3-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Gal-α-1,3-Gal-β-1,4-Glc-Sp5 GalNAc-β-1,3-Gal-α-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-a-2,3-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,3-Gal-β-1,4-Glc-Sp5 Neu5Gc-a-2,3-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,3-Gal-β-1,4-Glc-Sp5 Kdn-a-2,3-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,3-Gal-β-1,4-Glc-Sp5 Human Milk Oligosaccharides GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-(Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-GlcNAc-β-1,3-(Fuc-α-1,2-Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-[Fuc-α-1,2-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,6-]Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-(Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-GlcNAc-β-1,3-(Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-[Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,6-]Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-(Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-(Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-[Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,6-]Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-GlcNAc-β-1,3-(Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-(Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-(Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-[Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,6-]Gal-β-1,4-Glc-Sp5 Gal-β-1,4-Glc-Sp GalNAc-β-1,3-Gal-β-1,4-Glc-Sp Neu5Ac-α-2,3-Gal-β-1,4-Glc-Sp Neu5Gc-α-2,3-Gal-β-1,4-Glc-Sp Neu5Ac-α-2,6-Gal-β-1,4-Glc-Sp Neu5Gc-α-2,6-Gal-β-1,4-Glc-Sp Gal-α-1,3-Gal-β-1,4-Glc-Sp Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-Glc-Sp Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-Glc-Sp Neu5Ac-α-2,3-Gal-α-1,3-Gal-β-1,4-Glc-Sp Neu5Ac-α-2,6-Gal-α-1,3-Gal-β-1,4-Glc-Sp Gal-α-1,4-Gal-β-1,4-Glc-Sp5 GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,3-GalNAc-β-1,3-Gal-α-1,4-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-(Neu5Ac-α-2,6-)Gal-β-1,4-Glc-Sp5 Fuc-α-1,2-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Neu5Ac-α-2,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-Gal-β-1,4-(Fuc-α-1,3-)GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-Glc-Sp5 Gal-β-1,3-GlcNAc-β-1,3-(GlcNAc-β-1,6-)Gal-β-1,4-Glc-Sp5 Tandem Epitopes Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-Man-α-1,3-( Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Ac-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Gc-α-2,8-Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,8-Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Ac-α-2,8-Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,8-Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Gc-α-2,8-Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Ac-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Gc-α-2,8-Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Ac-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-( Neu5Gc-α-2,8-Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Neu5Ac-α-2,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Neu5Gc-α-2,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Neu5Gc-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(Neu5AC-α-2,6-)Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-[Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(Neu5AC-α-2,6-)Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(Neu5GC-α-2,6-)Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-[Neu5Gc-α-2,6-Gal-β-1,4-GlcNAc-β-1,3-(Neu5GC-α-2,6-)Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-(Neu5AC-α-2,6-)Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-[Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-(Neu5AC-α-2,6-)Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Neu5Ac-α-2,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-α-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,3-[Gal-α-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,3-Gal-β-1,4-(Fucα-1,3-)GlcNAc-β-1,2-Man-α-1,6-]Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,3-(Gal-α-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,4-GlcNAc-β-1,2-Man-α-1,6-)Man-β-1,4-GlcNAc-β-1,4-GlcNAc-β-Asn Application Data Kit Components Dialysis Vials Labeling Reagent Stop Solution Glycan Array Glass Slide Assembly Sample Diluent 20X Wash Buffer I 20X Wash Buffer II Cy3 equivalent dye-conjugated Streptavidin Slide Washer/Dryer Adhesive device sealer Labeling Buffer Floating Dialysis Rack Other Materials Required Detection antibodies of interest (For sandwich-based method only) Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Distilled or de-ionized water 1.5ml Polypropylene microcentrifuge tubes Gene microarray scanner or similar laser fluorescence scanner Small plastic or glass containers KCl, NaCl, KH2PO4 and Na2HPO4 (For label-based method only) Beaker, stir plate and stir bar Protocol Outline Dry the glass slide Block array surfaces Incubate samples (samples need to be biotinylated for the label-based approach) For the the sandwich-based principle, incubate with a detection antibody cocktail For the label-based principle, incubate the labeled-streptavidin. Incubate with Cy3 Equivalent Dye-Streptavidin Disassemble the glass slide Scan with a  gene microarray laser scanner Perform densitometry and analysis Storage/Stability Upon receipt, all components of the RayBiotech Glycan Array 300 kit should be stored at -20°C. After initial use, remaining reagents should be stored at 4°C to avoid repeated freeze-thaw cycles and may be stored for up to 3 months (Labeling Reagent, Item B, should be prepared fresh each time before use). Unused glass slides should be kept at -20°C and repeated freeze-thaw cycles should be avoided (slides may be stored for up to 6 months). The entire kit should be used within 6 months of purchase. References Amonsen, M.; Smith, D. F.; Cummings, R. D.; Air, G. M. J Virol 2007, 81, 8341-8345. Paulson, J. C.; de Vries, R. P. Virus Res 2013, 178, 99-113. Stencel-Baerenwald, J. E.; Reiss, K.; Reiter, D. M.; Stehle, T.; Dermody, T. S. Nat Rev Microbiol 2014, 12, 739-749. Park, S.; Shin, I. Org Lett 2007, 9, 1675-1678. Pourceau, G.; Chevolot, Y.; Goudot, A.; Giroux, F.; Meyer, A.; Moules, V.; Lina, B.; Cecioni, S.; Vidal, S.; Yu, H.; Chen, X.; Ferraris, O.; Praly, J. P.; Souteyrand, E.; Vasseur, J. J.; Morvan, F. Chembiochem 2011, 12, 2071-2080. Laurent, N.; Voglmeir, J.; Flitsch, S. L. Chem Commun 2008, 4400-4412. Xu, R.; de Vries, R. P.; Zhu, X.; Nycholat, C. M.; McBride, R.; Yu, W.; Paulson, J. C.; Wilson, I. A. Science 2013, 342, 1230-1235. Xu, R.; McBride, R.; Nycholat, C. M.; Paulson, J. C.; Wilson, I. A. J Virol 2012, 86, 982-990. Stevens, J.; Chen, L. M.; Carney, P. J.; Garten, R.; Foust, A.; Le, J.; Pokorny, B. A.; Manojkumar, R.; Silverman, J.; Devis, R.; Rhea, K.; Xu, X.; Bucher, D. J.; Paulson, J. C.; Cox, N. J.; Klimov, A.; Donis, R. O. J Virol 2010, 84, 8287-8299. Stowell, S. R.; Arthur, C. M.; Mehta, P.; Slanina, K. A.; Blixt, O.; Leffler, H.; Smith, D. F.; Cummings, R. D. J Biol Chem 2008, 283, 10109-10123. Stowell, S. R.; Arthur, C. M.; Slanina, K. A.; Horton, J. R.; Smith, D. F.; Cummings, R. D. J Biol Chem 2008, 283, 20547-20559. Carlsson, S.; Oberg, C. T.; Carlsson, M. C.; Sundin, A.; Nilsson, U. J.; Smith, D.; Cummings, R. D.; Almkvist, J.; Karlsson, A.; Leffler, H. Glycobiology 2007, 17, 663-676. Lingwood, C. A. Cold Spring Harb Perspect Biol 2011; 3: a004788 Bode, L. Glycobiology 2012, 22, 1147–1162