Glyco-Kit

Glycosylation is a reaction in which a carbohydrate is attached to a hydroxyl or other functional group of another molecule. There are many examples of natural molecules that gain, lose or change properties when carbohydrates are attached. Hydroxylation, methylation, fluorination etc. are part of the everyday tool box for many chemists. Glycosylation is more unusual. Glycosylation chemistry is difficult. Molecules with glycosylatable functional groups (-OH, -COOH, -NH, -SH) often need to have some of those groups blocked so that the sugar can be put where it is needed. We take a natural approach. Glycosyltransferase enzymes can glycosylate almost any molecule that has an appropriate side group. They can often work without any need to block side groups and many will work stereo-specifically. By selecting the right enzyme we can also add multiple different sugars (glucose, galactose, xylose, glucuronic acid, rhamnose etc.). We can also optimize (“evolve”) an enzyme to achieve a desired profile. We’re happy to talk to you to see if glycosylation can work for your project. Get in touch by email info@glycodepot.com or use the request a quote form . We can’t predict perfectly which enzymes will work with your molecule. But we can advise you on whether GLyco-Kit is likely to work for you. Once we have found the enzyme(s) that makes the change that you need, we can provide its UGT sequences as well as the associated expression systems. In the box The enzymes are provided in four 96-well microtiter plates. Each well contains enough purified enzyme for about ten reactions. We can provide additional copies of each plate if you need them. For confirmation of initial hits, quantification of activity and structure elucidation you will need HPLC or LC-MS analysis. Detailed protocols are provided with the kit. Online and phone support is available. We can provide the identity and sequence of any of the enzymes along with an E. coli expression plasmid containing the encoding gene. We also offer related discovery and scale-up services