α-Gal Lewis X (Galα1-3Glcβ1-3(Fucα1-4)GlcNAc) 

α-Gal Lewis X (Galα1-3Glcβ1-3(Fucα1-4)GlcNAc)  is a branched trisaccharide combining α-Gal epitope with Lewis X core, naturally occurring in mammalian glycoconjugates and tumor-associated antigens, synthesized as white powder for xenoantigen research, cancer diagnostics, and immunology. This structure (Gal α1-3 linked to Glc β1-3(Fuc α1-4)GlcNAc) elicits strong anti-Gal antibody binding while mimicking selectin ligands, aiding in studies of inflammation, metastasis, and xenotransplantation rejection; commercially available >90% pure via enzymatic synthesis, stable lyophilized at -20°C for multi-year use, with key roles in pathogen adhesion inhibition and glycan array standards. Appearance White to off-white amorphous powder or lyophilized solid. Highly water-soluble (>50 g/L); no distinct melting point reported. Source Endogenous in porcine/xenogeneic tissues, human tumors, and select mucins. Microbial/enzymatic production using fucosyl-/galactosyl-transferases on Lewis X acceptors. Molecular Weight and Structure Molecular mass 489.47 g/mol, formula C₁₉H₃₄NO₁₅ (core trisaccharide). Galα1-3Glcβ1-3(Fucα1-4)GlcNAc with branched α-Gal on reducing GlcNAc arm. Sugar Specificity Recognized by anti-α-Gal IgM/G, DC-SIGN, and selectins via Galα1-3 and Fucα1-4. Cleaved by α1-3 galactosidases and α1-4 fucosidases from Bifidobacterium spp. Biological Activity Triggers hyperacute rejection in xenotransplants; promotes tumor metastasis via E-selectin. Inhibits bacterial adhesion (e.g., H. pylori); modulates inflammation and α-Gal syndrome. Purity and Microbial Contamination 90-95% by NMR/HPLC; endotoxins <10 EU/mg. Pathogen-free, heavy metals < ICH limits, moisture <5% lyophilized. Identity and Quality Control ¹H-NMR for linkage confirmation; MALDI-TOF MS for mass; HPAEC for purity. Monosaccharide analysis by GC-MS; COA per batch. Shelf Life and Storage 2-3 years at -20°C lyophilized under argon. Short-term at 4°C; protect from moisture/hydrolases. Applications Xenograft antigen standards, cancer biomarker assays, selectin inhibitors. Glycan microarrays, vaccine design, anti-metastatic therapeutics. Key Characteristics Acid-stable to stomach; colonic utilization; dual epitope for precise targeting. Citation Links https://pubchem.ncbi.nlm.nih.gov/compound/22806751 https://www.elicityl-oligotech.com/xeno-antigens/447-xeno-lewisx--a-gal-lewisx.html https://www.amerigoscientific.com/xeno-lewis-x-gal-lewis-x-grafted-on-bsa-item-488928.html https://pmc.ncbi.nlm.nih.gov/articles/PMC6194003/ https://www.vulcanchem.com/product/vc212695 https://pmc.ncbi.nlm.nih.gov/articles/PMC2271034/ https://pubchem.ncbi.nlm.nih.gov/compound/22806751 https://www.qa-bio.com/product/alpha1-3-gal-standard/ https://pmc.ncbi.nlm.nih.gov/articles/PMC7583601/ https://glycosmos.org/structure/alpha-Gal-LewisX