GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAc

GlcNAcβ1-3Galβ1-4(Fucα1-3)GlcNAc  (difucosyl poly-LacNAc type 2 trisaccharide, branched Le^x-GlcNAc) appears as a white lyophilized powder critical for selectin ligand research, tumor metastasis studies, and granulocyte function assays. This branched trisaccharide features GlcNAcβ1-3 extending from fucosylated LacNAc [Galβ1-4(Fucα1-3)GlcNAc], serving as core structure for extended sialyl-Lewis X chains while binding monoclonal antibodies (WEM-G1) specific to human neutrophils/eosinophils; synthesized chemoenzymatically via β3GnT2/α1-3FucT cascades (>98% purity), it mediates leukocyte rolling via E/P-selectin interaction, exhibits gastric stability with selective Bacteroides fucosidase hydrolysis, and plays key roles in cancer glycomimetics, anti-inflammatory drug screening, and functional food prebiotics targeting Akkermansia growth, demonstrating multi-year stability for glycan microarray applications. Appearance White amorphous lyophilized powder, odorless, non-hygroscopic. Water solubility >300 g/L; clear solutions pH 6-8. ​ Source Extended poly-LacNAc chains on human leukocytes, tumor mucins. Biosynthetic via β1-3GlcNAc transferase on Lewis X (Galβ1-4(Fucα1-3)GlcNAc). ​ Molecular Weight and Structure 613.6 g/mol, formula C₂₄H₄₂N₂O₁₈ (sodium salt). Branched: GlcNAcβ1-3[Galβ1-4(Fucα1-3)GlcNAc] Le^x core extension. ​ Sugar Specificity E/P-selectin ligands; WEM-G1 mAb binding to neutrophils/eosinophils. Hydrolyzed by Bacteroides α1-3 fucosidase + β3GlcNAc-ase. ​ Biological Activity Mediates granulocyte rolling/ADCC; precursor for sLeX tumor antigens. Prebiotic for Akkermansia muciniphila; anti-metastatic potential. ​ Purity and Microbial Contamination ≥98% HPLC/NMR; endotoxins <5 EU/mg LAL-tested. Sterile filtered; heavy metals <1 ppm; moisture <4%. ​ Identity and Quality Control ¹H-NMR (Fuc H1 5.1 ppm α, GlcNAc H1 4.7 ppm β); LC-MS [M+Na]⁺ 636.4. HPAEC Gal:GlcNAc:Fuc 1:2:1; anomeric purity >99%. ​ Shelf Life and Storage 48 months -20°C lyophilized; 24 months ambient desiccated. Protect from humidity; N₂ atmosphere preferred. ​ Applications Selectin inhibitor screening; granulocyte function assays. Tumor metastasis biomarkers; glycoengineering scaffolds. ​ Key Characteristics Branched Le^x specificity; high avidity selectin binding. Core scaffold for poly-sLeX chains; gastric-resistant. ​ Citation Links https://ww w.vulcanchem.com/product/vc20916493 ​ https://pubchem.ncbi.nlm.nih.gov/compound/3083489 ​ https://cymitquimica.com/products/3B-G0465/77735-22-9/glcnac1-4fuc1-3glcnac/ ​ https://www.bioglyco.com/gal1-3gal1-4fuc1-3glcnac-sp3-paa-item-5159.html ​ https://doi.org/10.1074/jbc.271.31.18732 ​ https://academic.oup.com/glycob/article/15/10/943/693676 ​ https://pubchem.ncbi.nlm.nih.gov/compound/FGJTXYWKOHAINP-ZZSHMEFKSA-N ​ https://glycosmos.org/structure/Le^x-GlcNAc ​ https://www.cd-bioglyco.com/glcnac-beta1-3-gal-beta1-4-fuc-alpha1-3-glcnac ​ https://www.sciencedirect.com/science/article/pii/S0021925820854969 ​