Y‑GlcA CHX Salt 

Y‑GlcA CHX Salt (CAS 216971‑58‑3), systematically 3‑indolyl‑β‑D‑glucuronic acid cyclohexylammonium salt, is a synthetic β‑D‑glucuronidase‑specific chromogenic substrate in which a 3‑indolyl (indoxyl) aglycone is linked via a β‑glycosidic bond to β‑D‑glucuronic acid and presented as the cyclohexylammonium salt. When hydrolyzed by β‑D‑glucuronidase (EC 3.2.1.31, GUS), the indoxyl moiety is released and oxidizes to form an insoluble yellow‑to‑brownish precipitate, enabling clear visual detection of enzyme activity. The CHX salt form is highly soluble in organic solvents such as DMSO or DMF, allowing preparation of concentrated stock solutions that are then diluted into aqueous media for use in bacterial‑colony screening, GUS‑reporter assays, and histochemical staining of β‑glucuronidase activity in plant and animal tissues. The yellow‑brown hue provides good contrast with blue‑colored backgrounds and can be combined with blue‑forming substrates in multiplex‑detection systems. Y‑GlcA CHX Salt is supplied as a high‑purity, research‑use‑only reagent compatible with standard microbiological and molecular‑biology buffers.

• Shenzhen GeneSeqTools – Y‑GlcA CHX Salt product page (CAS 216971‑58‑3, MF C₂₀H₂₈N₂O₇, MW 408.45, freeze‑dried powder): http://www.geneseqtools.cn/cn/product03_cid_215_pid_546.html
• Hepattack Bioscience – Y‑GlcA CHX Salt product listing (CAS 216971‑58‑3, high‑purity raw material for research): https://www.hepattack.com/en/product03_cid_215_pid_546.html
• GeneSeqTools news page (Y‑GlcA CHX Salt details, CAS 216971‑58‑3, freeze‑dried powder, 1 g/5 g/25 g): http://www.geneseqtools.com/cn/news-detail_cid_26_id_118.html
• GBiosciences – 3‑Indoxyl‑β‑D‑glucuronic acid cyclohexylammonium salt (Y‑GlcA CHX‑class substrate): https://www.gbiosciences.com/Bioassays/Enzyme-Substrates/3-Indoxyl-beta-D-glucuronic-acid-cyclohexylammonium-salt
• 3‑Indolyl‑β‑D‑glucuronide sodium salt product page (for dye‑chemistry and β‑glucuronidase‑substrate context): https://www.biosynth.com/p/EI03299/119736-51-5-3-indolyl-b-d-glucuronide-sodium-sal
• Sigma‑Aldrich – X‑GlcA (5‑bromo‑4‑chloro‑3‑indolyl‑β‑D‑glucuronide cyclohexylammonium salt) entry (for CHX‑salt‑type GUS‑substrate structure and assay‑use): https://www.sigmaaldrich.com/US/en/product/mm/203783
• Melford – X‑GlcA Cyclohexylammonium Salt product page (for GUS‑substrate‑handling and storage reference): https://www.melford.co.uk/products/biochemicals/enzyme-substrates/x-glca-cyclohexylammonium-salt-1-g.html
• Molecular Depot – X‑gluc (CHX salt) entry (for CHX‑salt‑class chromogenic‑substrate principles): https://moleculardepot.com/product/x-gluc-chx-salt/
• β‑Glucuronidase‑substrate overview (ScienceDirect or review‑style chromogenic‑substrate topic): https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/chromogenic-substrate
• General glycosidase‑substrate catalog (Y‑GlcA and related indolyl‑β‑D‑glucuronides overview): https://www.chemicalbook.com/ (search for “216971‑58‑3”)